>AGAP012068-PA

gnl|CDD|64510 pfam00648, Peptidase_C2, Calpain family cysteine p...   433   e-122
gnl|CDD|64906 pfam01067, Calpain_III, Calpain large subunit, dom...   223   3e-059
gnl|CDD|70679 pfam07223, DUF1421, Protein of unknown function (D...    34   0.055
gnl|CDD|63925 pfam00036, efhand, EF hand. The EF-hands can be di...    33   0.077
gnl|CDD|68080 pfam04495, GRASP55_65, GRASP55/65 family. GRASP55 ...    32   0.14 
gnl|CDD|65230 pfam01416, PseudoU_synth_1, tRNA pseudouridine syn...    31   0.23 
gnl|CDD|66806 pfam03154, Atrophin-1, Atrophin-1 family. Atrophin...    30   0.66 


==> gnl|CDD|64510 pfam00648, Peptidase_C2, Calpain family cysteine protease..
          Length = 303

 Score =  433 bits (1116), Expect = e-122
 Identities = 177/308 (57%), Positives = 222/308 (72%), Gaps = 12/308 (3%)

Query: 151 LFEDPEFPASASSLMYSKRPD-----RHYEWLRPHEICDGPEFFVEGFSRFDVQQGELGD 205
           LF DP FPA   SL Y          R  EW RPHEIC+ P+F + G +R D+ QG LGD
Sbjct: 1   LFVDPSFPADPKSLGYKPLGPYSSKTRGIEWKRPHEICENPQFIIGGATRTDICQGALGD 60

Query: 206 CWLLAACANLTQDHKMFLRVVPEDNSFEDGYAGVFHFRFWQYGKWVDVVIDDRLPTYRGQ 265
           CWLLAA A+LT + ++  RVVP D SF++ YAG+FHF+FWQYG+WVDVV+DDRLPT  G+
Sbjct: 61  CWLLAAIASLTLNEELLFRVVPHDQSFQENYAGIFHFQFWQYGEWVDVVVDDRLPTKDGE 120

Query: 266 LIYMRSSEQNEFWSALLEKAYAKLHGSYESLRGGTTCEAMEDFTGGVAEMYDLKDQTPP- 324
           L+++ S+E+NEFWSALLEKAYAKLHG YE+L GG+T EAMEDFTGGVAE Y+LK      
Sbjct: 121 LVFVHSAERNEFWSALLEKAYAKLHGCYEALSGGSTTEAMEDFTGGVAEWYELKQAPSDL 180

Query: 325 NLFEIIEKGFKRNSMFGCSI-EADPHVLEAETPEGLIRGHAYSITKIQLVDIETPGRSGK 383
           NLF+II K  +R S+ GCSI    P  +EA T +GL++GHAYS+T ++ V+     R  K
Sbjct: 181 NLFKIIAKALERGSLMGCSIDITSPVDMEARTFKGLVKGHAYSVTGVKEVN----YRGEK 236

Query: 384 IPLIRLRNPWGNEAEWNGPWSDKSPEWRYIPDEQKQELGLNFDHDGEFWMSYRDFTRYFD 443
             LIRLRNPWG + EW G WSD SPEWR+I  +++  L L F+ DGEFWMS+ DF R+F 
Sbjct: 237 QKLIRLRNPWG-QVEWTGAWSDGSPEWRFIDPDERARLQLQFEEDGEFWMSFEDFLRHFS 295

Query: 444 RMEICNLS 451
           R+EICNL+
Sbjct: 296 RLEICNLT 303



==> gnl|CDD|64906 pfam01067, Calpain_III, Calpain large subunit, domain III. The
           function of the domain III and I are currently unknown.
           Domain II is a cysteine protease and domain IV is a
           calcium binding domain. Calpains are believed to
           participate in intracellular signaling pathways mediated
           by calcium ions..
          Length = 150

 Score =  223 bits (570), Expect = 3e-059
 Identities = 92/147 (62%), Positives = 113/147 (76%), Gaps = 2/147 (1%)

Query: 466 SWEMSMFEGEWAVGTTAGGCRNYLDTFWHNPQYVIRLDDPDEDDEEGNCTVIIALLQKNR 525
            WE ++ EGEW  G+TAGGCRNY DTFW NPQY+I L++PD+DD++  C+V++AL+QK+R
Sbjct: 1   KWEETIVEGEWTRGSTAGGCRNYPDTFWTNPQYIITLEEPDDDDDDSECSVLVALMQKDR 60

Query: 526 RSRRNMGVECLTIGFAVYRVTERDLAQKPLKMNFFKYNASAARSPAFINLREVSCRFKLP 585
           R  R MG + LTIGFAVY+V +       L  +FF  N S ARS  FINLREVS RF+LP
Sbjct: 61  RRERRMGEDMLTIGFAVYKVPDEL--NVHLSRDFFLTNQSRARSSTFINLREVSLRFRLP 118

Query: 586 PGTYVIVPSTFEPNEEGEFIIRVFSET 612
           PG YV+VPSTFEPNEEGEFI+RVFSE 
Sbjct: 119 PGEYVVVPSTFEPNEEGEFILRVFSEK 145



==> gnl|CDD|70679 pfam07223, DUF1421, Protein of unknown function (DUF1421). This
           family represents a conserved region approximately 350
           residues long within a number of plant proteins of
           unknown function..
          Length = 331

 Score = 33.6 bits (76), Expect = 0.055
 Identities = 30/116 (25%), Positives = 36/116 (31%), Gaps = 27/116 (23%)

Query: 6   QSPPA-SQWPTVPTPGSSGYSSLNPSISGQSLSYGFNPNVMP---ALPDSGAGYP----- 56
           Q+PP   Q+P  P P  SGY+        Q  SY  N N  P     P S          
Sbjct: 129 QAPPQQPQYPQQP-PPPSGYNPEEQPAQTQ--SYPPNQNWPPQPQPPPGSSPSQQTYNPP 185

Query: 57  ---PAGNQLP--YPTMGFAGFGSPMDYT----------MPNLPPPVPDALLRGAEP 97
              P+    P      GF    S   Y             +  PP P    +GA P
Sbjct: 186 PPQPSMYDGPGGRSNSGFPSGYSMEAYGYGGAPPPQAGRMSYKPPHPSQQGQGAYP 241



==> gnl|CDD|63925 pfam00036, efhand, EF hand. The EF-hands can be divided into two
           classes: signaling proteins and buffering/transport
           proteins. The first group is the largest and includes
           the most well-known members of the family such as
           calmodulin, troponin C and S100B. These proteins
           typically undergo a calcium-dependent conformational
           change which opens a target binding site. The latter
           group is represented by calbindin D9k and do not undergo
           calcium dependent conformational changes..
          Length = 29

 Score = 32.9 bits (76), Expect = 0.077
 Identities = 9/28 (32%), Positives = 14/28 (50%)

Query: 691 CRAMVAMLDVDHTGKLGFEEFQQLLTDI 718
            +      D D  GK+ FEEF++ L  +
Sbjct: 2   LKEAFKEFDKDGDGKISFEEFKEALKKL 29



==> gnl|CDD|68080 pfam04495, GRASP55_65, GRASP55/65 family. GRASP55 (Golgi reassembly
           stacking protein of 55 kDa) and GRASP65 (a 65 kDa)
           protein are highly homologous. GRASP55 is a component of
           the Golgi stacking machinery. GRASP65, an
           N-ethylmaleimide- sensitive membrane protein required
           for the stacking of Golgi cisternae in a cell-free
           system..
          Length = 321

 Score = 32.0 bits (72), Expect = 0.14
 Identities = 19/99 (19%), Positives = 26/99 (26%), Gaps = 4/99 (4%)

Query: 1   PAAFAQSPPASQWPTVPTPGSSGYSSLNPSISGQSLSYGFNPNVMPALPDSGAG--YPPA 58
           PAA     P+        P +   SS+                     P        PP 
Sbjct: 153 PAATNFPDPSPLLSPFSAPPAP--SSVLQLGGPTPPYPPPLVQQGSYAPPVEPETQLPPP 210

Query: 59  GNQLPYPTMGFAGFGSPMDYTMPNLPPPVPDALLRGAEP 97
            + L +     A  GS  +   P   P  P      A+P
Sbjct: 211 SSLLGHGLSSSANAGSSNEIPPPAPQPTPPYVQSPSAQP 249



==> gnl|CDD|65230 pfam01416, PseudoU_synth_1, tRNA pseudouridine synthase. Involved
           in the formation of pseudouridine at the anticodon stem
           and loop of transfer-RNAs Pseudouridine is an isomer of
           uridine (5-(beta-D-ribofuranosyl) uracil, and id the
           most abundant modified nucleoside found in all cellular
           RNAs. The TruA-like proteins also exhibit a conserved
           sequence with a strictly conserved aspartic acid, likely
           involved in catalysis..
          Length = 101

 Score = 31.4 bits (71), Expect = 0.23
 Identities = 21/73 (28%), Positives = 29/73 (39%), Gaps = 8/73 (10%)

Query: 662 EVDWVELKRILDHSFRDDNISSEGFSKDVCRAMVAMLDVDHTGKLGFEEFQQLLT----- 716
            +    L RI     R   IS  GF+    RA+V +L     G+   E+ ++LL      
Sbjct: 26  TILEKALSRIGGEKIR---ISGAGFTDKGVRAIVQVLLFVGPGERPPEDIKELLNAKLRD 82

Query: 717 DIAKWKAVFKLYD 729
           DI    A   LY 
Sbjct: 83  DIGVLTAPAGLYL 95



==> gnl|CDD|66806 pfam03154, Atrophin-1, Atrophin-1 family. Atrophin-1 is the protein
           product of the dentatorubral-pallidoluysian atrophy
           (DRPLA) gene. DRPLA OMIM:125370 is a progressive
           neurodegenerative disorder. It is caused by the
           expansion of a CAG repeat in the DRPLA gene on
           chromosome 12p. This results in an extended
           polyglutamine region in atrophin-1, that is thought to
           confer toxicity to the protein, possibly through
           altering its interactions with other proteins. The
           expansion of a CAG repeat is also the underlying defect
           in six other neurodegenerative disorders, including
           Huntington's disease. One interaction of expanded
           polyglutamine repeats that is thought to be pathogenic
           is that with the short glutamine repeat in the
           transcriptional coactivator CREB binding protein, CBP.
           This interaction draws CBP away from its usual nuclear
           location to the expanded polyglutamine repeat protein
           aggregates that are characteristic of the polyglutamine
           neurodegenerative disorders. This interferes with
           CBP-mediated transcription and causes cytotoxicity..
          Length = 984

 Score = 29.8 bits (66), Expect = 0.66
 Identities = 20/86 (23%), Positives = 29/86 (33%), Gaps = 3/86 (3%)

Query: 6   QSPPASQWPTVPTPGSSGYSSLNPSISGQSLSYGFNPNVMPALPDSGAGYPPAGNQLPYP 65
           QSP A        PG+    ++ PSI   S  +   P             P   +  P+ 
Sbjct: 210 QSPTAPPPDVPQQPGAQSVPAVAPSIQAASALHPQRPPSPHNPLSPFLFPPSLPSAQPHA 269

Query: 66  TMGFAGFGSPMDYTM---PNLPPPVP 88
                G G P  +++   P L  P P
Sbjct: 270 QPPLHGQGPPSPHSLQAGPLLQHPQP 295



 Score = 29.4 bits (65), Expect = 0.87
 Identities = 19/90 (21%), Positives = 26/90 (28%), Gaps = 4/90 (4%)

Query: 1   PAAFAQSPPASQ----WPTVPTPGSSGYSSLNPSISGQSLSYGFNPNVMPALPDSGAGYP 56
           P    Q PP+       P +  P          +  GQ+          P+     +  P
Sbjct: 271 PPLHGQGPPSPHSLQAGPLLQHPQPPQNFLPPQASQGQAPLGTSPAAAYPSQSALQSQQP 330

Query: 57  PAGNQLPYPTMGFAGFGSPMDYTMPNLPPP 86
           P    LP    G      P    +P LP P
Sbjct: 331 PREQPLPPAPSGMPHIKPPPTTPIPQLPAP 360