Bibliography: Chris Hughes
Original Research Articles:
1. Hughes CCW, Lantos PL. 1986. Brain capillary endothelial cells in vitro lack surface IgG Fc receptors. Neurosci. Lett. 68:100-106
2. Hughes CCW, Lantos PL. 1987. A morphometric study of blood vessel, neuron and glial cell distribution in young and old rat brain. J. Neurol. Sci. 79:101-110
3. Male DK, Pryce G, Hughes CCW. 1987. Antigen presentation in brain: MHC induction on brain endothelium and astrocytes compared. Immunology 60:453-459
4. Hughes CCW, Male DK, Lantos PL. 1988. Adhesion of lymphocytes to cerebral microvascular cells: Effects of interferon-_, tumour necrosis and interleukin-1. Immunology 64:677-681
5. Hughes CCW, Lantos PL. 1989. Uptake of leucine and alanine by cultured cerebral capillary endothelial cells. Brain Res. 480:126-132
6. Roux FS, Mokni R, Hughes CCW, Clouet PM, LeFauconnier J-M, Bourre J-M. 1989. Lipid synthesis by rat brain microvessel endothelial cells in tissue culture. J. Neuropath. Exp. Neurol. 48:437-447
7. Male D, Pryce G, Hughes CCW, Lantos P. 1990. Lymphocyte migration into brain modelled in vitro: Control by lymphocyte activation, cytokines, and antigen. Cell. Immunol. 127:1-11
8. Begley DJ, Squires LK, Zlokovic BV, Mitrovic DM, Hughes CCW, Revest PA, Greenwood J. 1990. Permeability of the blood-brain barrier to the immunosuppressive cyclic peptide Cyclosporin A. J. Neurochem. 55:1222-1230
9. Sedgwick JD, Hughes CCW, Male DK, MacPhee IAM, ter Meulen V. 1990. Antigen-specific damage to brain vascular endothelial cells mediated by encephalitogenic and nonencephalitogenic CD4+ T cell lines in vitro. J.Immunol.145:2474-2481
10. Hughes CCW, Savage COS, Pober JS. 1990. Endothelial cells augment T cell IL-2 production by a contact-dependent mechanism involving CD2:LFA-3 interaction. J. Exp. Med. 171:1453-1467
11. Salomon RN, Hughes CCW, Schoen FJ, Payne DD, Pober JS, Libby P. 1991. Human coronary transplantation-associated arteriosclerosis: evidence for a chronic immune reaction to activated graft endothelial cells. Am. J. Path. 138:791-798
12. Savage COS, Hughes CCW, Pepinsky RB, Wallner BP, Freedman AS, Pober JS. 1991. Endothelial cell lymphocyte function-associated antigen-3 and an unidentified ligand act in concert to provide costimulation to human peripheral blood CD4+ T cells. Cell. Immunol. 137: 150-163
13. Abbott NJ, Hughes CCW, Revest PA and Greenwood J. 1992. Development and characterisation of a rat brain capillary endothelial culture: towards an in vitro blood-brain barrier. J. Cell. Sci. 103: 23-37
14. Hughes CCW, Savage COS*, McIntyre BW and Pober JS. 1992. Human CD4+ T cells proliferate to HLA-DR+ allogeneic vascular endothelium: identification of accessory interactions. Transplantation 56: 128-134 * joint first author.
15. Hughes CCW and Pober JS. 1992. Costimulation of peripheral blood T cell activation by human endothelial cells: enhanced IL-2 transcription correlates with increased c-fos synthesis and increased Fos levels in AP-1. J. Immunol. 150:3148-3160
16. Murray AM, Petzelbauer P, Hughes CCW, Costa J, Askenase P and Pober JS. 1994. Human T cell-mediated destruction of allogeneic dermal microvessels in a severe combined immunodeficient mouse. Proc. Natl. Acad. Sci. 91: 9146-9150
17. Karmann K, Pober JS and Hughes CCW. 1994. Endothelial cell-induced resistance to cyclosporin A in human peripheral blood T cells requires contact-dependent interactions involving CD2 but not CD28. J. Immunol. 153: 3929-3937
18. Karmann K, Hughes CCW, Schechner J, Fanslow WC and Pober JS. 1995. CD40 on human endothelial cells: Inducibility by cytokines and functional regulation of adhesion molecule expression. Proc. Natl. Acad. Sci. 92: 4342-4346
19. Chang CH, Hong SC, Hughes CCW, Janeway CA, Jr., and Flavell RA. 1995. CIITA activates the expression of MHC class II genes in mouse T cells. Int. Immunol. 7: 1515-8
20. Hughes CCW and Pober JS. 1996. Transcriptional regulation of the Interleukin 2 gene in normal human peripheral blood T cells: convergence of costimulatory signals and differences from transformed T cells. J. Biol. Chem., 271: 5369-5377
21. Palmer-Crocker RL, Hughes CCW and Pober JS. 1996. IL-4 and IL-13 activate the Jak2 tyrosine kinase and Stat6 in cultured human vascular endothelial cells through a common pathway that does not involve the _c chain. J. Clin. Invest., 98: 604-609
22. Karmann K, Hughes CCW, Fanslow WC and Pober JS. 1996. Endothelial cells augment the expression of CD40L on newly activated human CD4+ T cells through a CD2/LFA-3 signaling pathway. Eur. J. Immunol., 26: 610-617
23. Maher SE, Karmann K, Min W, Hughes CCW, Pober JS and Bothwell ALM. 1996. Porcine endothelial CD86 is a major costimulator of xenogeneic human T cells: Cloning, sequencing and functional expression in human endothelial cells. J. Immunol. 157: 3838-3844
24. Wang W, Tam WF, Hughes CCW, Rath S, and Sen R. 1997. c-Rel is a target of pentoxifylline-mediated inhibition of T cell activation. Immunity 6: 165-174
25. Sultan P, Shechner J, McNiff JM, Hochman PS, Hughes CCW, Lorber MI, Askenase PW and Pober JS. 1997. Blockade of CD2-LFA-3 interactions protects human skin allografts in immunodeficient mouse/human chimeras. Nature Biotech. 15: 759-762
26. Cron RQ, Schubert LA, Lewis DB, and Hughes CCW. 1997. Protocols for consistent transient transfections of DNA into normal human and murine T lymphocytes. J. Immunol. Methods, 205: 145-150
27. Gonsky R, Deem RL, Hughes CCW, and Targan SR. 1998. Activation of the CD2 pathway in Lamina Propria T cells up-regulates functionally active AP-1 binding to the IL-2 promoter, resulting in mRNA transcription and IL-2 secretion. J. Immunol. 160: 4914-4922
28. Murray AG, Shechner J, Epperson DE, Sultan P, McNiff JF, Hughes CCW, Lorber MI, Askenase PW and Pober JS. 1998. Dermal microvascular injury in the human peripheral blood lymphocyte reconstituted-Severe Combined Immunodeficient (Hu-PBL-SCID) mouse/skin allograft model is T cell-mediated and inhibited by a combination of cyclosporine and rapamycin. Am. J. Pathol. 153: 627-638
29 Salazar Murphy LL, Mazanet MM, Taylor AC, Mestas J, and Hughes CCW. 1999 Single-cell analysis of costimulation by B cells, endothelial cells, and fibroblasts demonstrates heterogeneity in responses of CD4+ memory T cells. Cell. Immunol. 194: 150-161 (Pdf)
30. Taylor JA, Tewari K, Liao S-Y, Hughes CCW* and Villarreal LP. 1999 Immunohistochemical analysis, human papillomavirus DNA detection, hormonal manipulation, and exogenous gene expression of normal and dysplastic human cervical epithelium in severe combined immunodeficiency mice. J. Virol. 73: 5144-5148 * Joint senior author.
31. Mazanet MM, Neote K and Hughes CCW 2000. Expression of Interferon-inducible T cell alpha chemoattractant (I-TAC) by human endothelial cells is CsA-resistant and promotes T cell adhesion: Implications for CsA-resistant immune inflammation. J. Immunol. 164: 5383-88 (Pdf)
32. Tewari K, Taylor JA, Liao S-Y, DiSaia PJ, Burger RA, Monk BJ, Hughes CCW* and Villarreal LP 2000 Development and assessment of a general theory of cervical carcinogenesis utilizing a severe combined immunodeficiency murine-human xenograft model. Gynecol. Oncol. 77: 137-148 * Joint senior author.
33. Schechner JS, Nath A, Zheng L, Kluger MS, Hughes CCW, Sierra-Honigmann MDL, Lorber MI, Tellides G, Kashgarian M, Bothwell ALM and Pober JS 2000. In vivo formation of complex microvessels lined by human endothelial cells in an immunodeficient mouse. PNAS 97: 9191-9196
34. Henderson AM, Wang S-J, Taylor AC, Aitkenhead M and Hughes CCW 2001 The bHLH transcription factor HESR1 regulates endothelial cell tube formation. J. Biol. Chem. 276: 6169-6176 (Pdf)
35. Mestas J and Hughes CCW. 2001. Endothelial cell costimulation of T cell activation through CD2-CD58 interactions involves lipid-raft aggregation J. Immunol. 167: 4378-4385 (Pdf)
36. Aitkenhead M, Wang S-J, Mestas J, Heard C and Hughes CCW. 2002. Identification of endothelial cell genes expressed in an in vitro model of angiogenesis: upregulation of ESM-1, big-h3 and NrCAM. Microvascular Research. 63: 159-171 (Pdf)
37. Mazanet MM and Hughes CCW. 2002. B7-H1 expression by activated endothelial cells mediates costimulation of T cell IL-10 production J. Immunol. 169: 3581-3588 (Pdf)
38. Salazar Murphy LL and Hughes CCW. 2002. Endothelial cells stimulate NFAT nuclear translocation in the presence of CsA: involvement of the wnt/GSK-3b pathway J. Immunol. 169: 3717-3725 (Pdf)
39. Taylor KT and Hughes CCW. 2002. Notch activation during endothelial cell tube formation in vitro targets the basic HLH transcription factor HESR1 and downregulates VEGFR-2/KDR expression. Microvascular Research. 64: 372-383 (Pdf)
40. Pedram A, Razandi M, Aitkenhead M, Hughes CCW and Levin ER. 2002 Integration of the non-genomic and genomic actions of estrogen: Membrane Initiated Signaling by Steroid (MISS) to transcription and cell biology. J. Biol. Chem. 277: 50768-50775 (Pdf)
41. Nakatsu MN, Sainson RCA, Aoto JN, Taylor KL, Aitkenhead M, Pérez del Pulgar S, Carpenter PM, and Hughes CCW. 2003 Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin-1. Microvascular Research 66: 102-112 (Pdf)
42. Nakatsu MN, Sainson RCA, Pérez del Pulgar S, Aoto JN, Aitkenhead M, Taylor KL, Carpenter PM and Hughes CCW. 2003 VEGF121 and VEGF165 regulate blood vessel diameter through VEGFR-2 in an in vitro angiogenesis model. Laboratory Investigation 83: 1873-1885. (Pdf)
43. Griffith GK, Miller C, Sainson RCA, Calvert JW, Jeon NL, Hughes CCW, and George SC. 2005. Diffusion limits of an in vitro thick prevascularized tissue. Tissue Eng. 11: 257-266.
44. Sainson RCA, Aoto J, Nakatsu MN, Holderfield M, Conn E, Koller E, and Hughes CCW. 2005 Re-iterative Notch signaling regulates endothelial cell branching and proliferation during vascular tubulogenesis FASEB J 19: 1027-9
45. Mestas, J, Hori T and Hughes CCW. 2005 Endothelial cell costimulation through OX40 prolongs T cell cytokine synthesis via p38-MAPK-mediated mRNA stabilization. Int. Immunol. 17: 737-47
46. Payvandi F, Wu L, Naziruddin S, Haley M, Parton A, Schafer PH, Chen RS, Muller GW, Hughes CCW, and Stirling DI. 2005. Immunomodulatory drugs (IMiDs) increase the production of IL-2 from stimulated T cells by increasing PKC Activation And Enhancing the DNA binding activity of AP-1 but not NF-kB, OCT-1 or NF-AT. J. Interferon & Cytokine Res. 25: 604-16
47. Holderfield MT, Henderson Anderson AM, Kokubo H, Chin MT, Johnson RL, and Hughes CCW. 2006 HESR1/CHF2 suppresses VEGFR2 transcription independent of binding to E-boxes. Biol. Biochem. Res. Comm. 346: 637-648
48. Ghajar CM, Blevins KS, Hughes CCW, George SC, and Putnam AJ. 2006 Mesenchymal stem cells enhance angiogenesis in mechanically viable prevascularized tissues via early MMP upregulation. Tissue Engineering 10: 2875-2888.
49. Wu B, Crampton SP, and Hughes CCW. 2007. Wnt signaling induces MMP expression and regulates T cell transmigration. Immunity 26: 227-239.
50. Pedram A, Razandi M, Sainson RC, Kim JK, Hughes CCW, and Levin ER. 2007. A conserved mechanism for steroid receptor translocation to the plasma membrane. J. Biol. Chem. 282: 22278-88.
51. Nakatsu MN, Davis J, and Hughes CCW 2007 An optimized fibrin gel bead assay for the study of angiogenesis in vitro. J. Vis. Exp. (JoVE) 3: (Apr) doi:10.3791/186
52. Ghajar CM, Harris JW, Suresh V, Chen X, Jeon NL, Hughes CCW, Putnam AJ, and George SC. 2007. The Effect of Matrix Density on the Regulation of 3-D Capillary Morphogenesis. Biophys. J. 94: 1930-41
53. Sainson RCA, Johnston DA, Chu HC, Holderfield MT, Nakatsu MN, Crampton SP, Davis J, Conn E, and Hughes CCW. 2008 TNF Primes Endothelial Cells for Angiogenic Sprouting by Inducing a Tip Cell Phenotype. Blood 111: 4997-5007.
54. Lu L, Payvandi F, Wu L, Zhang L-H, Hariri RJ, Man H-W, Chen RS, Muller GW, Hughes CCW, Stirling DI, Schafer PH, and Bartlett JB. 2009 The anti cancer drug lenalidomide inhibits angiogenesis and metastasis via multiple inhibitory effects on endothelial cell function in normoxic and hypoxic conditions. Microvasc. Res. 77: 78-86
55. Chen X, Aledia AS, Ghajar CM, Griffith CK, Putnam AJ, Hughes CCW, and George SC. 2009 Prevascularization of a fibrin-based tissue construct accelerates the formation of functional anastomosis with host vasculature. Tissue Engineering 15: 1363-71.
56. Johnston DA, Dong B, and Hughes CCW. 2009 Induction of jagged-1 in endothelial cells is NFkB dependent. Gene 435: 36-44.
57. Crampton SP, Wu B, Park E, Kim J-H, Solomon C, Waterman ML, and Hughes CCW. 2009. Integration of the _-catenin-dependent wnt pathway with integrin signaling through the adaptor molecule Grb2. PLoS One. 4: e7841
58. Chen X, Aledia AS, Popson SA, Him L, Hughes CCW* and George SC*. 2010. Rapid anastomosis of endothelial precursor cell-derived vessels with host vasculature is promoted by a high density of co-transplanted fibroblasts. Tissue Engineering 16: 585-94. * co-senior and corresponding authors
59. Baruscotti I, Barchiesi F, Jackson EK, Imthurn B, Stiller R, Kim JH, Schaufelberger S, Rosselli M, Hughes CCW, and Dubey RK. 2010. Estradiol stimulates capillary formation by human endothelial progenitor cells: role of estrogen receptor-{alpha}/{beta}, heme oxygenase 1, and tyrosine kinase. Hypertension. 56: 397-404
60. Popson SA, Holderfield MT, Shaaban C, and Hughes CCW. 2011 IFITM1 regulates endothelial cell sprouting and lumen formation during angiogenesis. (in preparation).
61. Newman AC, Phan D, Nakatsu MN, Cho W, Gershon PD, and Hughes CCW. 2011 Identification of fibroblast-derived proteins important for EC sprouting and lumen formation. Mol. Biol. Cell (in press).
62. Kim J-H, George SC, and Hughes CCW. 2011 Notch activation can overcome loss of ALK1 (HHT2) in regulation of endothelial cell EphrinB2 expression and angiogenic sprouting. (in preparation).
Review Articles:
1. Pober JS, Doukas J, Hughes CCW, Savage COS, Munro JM, Cotran RS. 1990. The potential roles of vascular endothelium in immune reactions. Human Immunol. 28:258-262
2. Hughes CCW, Savage COS, Pober JS. 1990. The endothelial cell as a regulator of T cell function. Immunol. Rev. 117:85-102
3. Mestas J, and Hughes CCW 2004. Of mice and not men: differences between mouse and human immunology. J. Immunol. 172: 2731-2738 (Pdf)
4. Holderfield MT and Hughes CCW. 2007 Crosstalk between VEGF, notch, and TGFb in vascular morphogenesis. Circ. Res 102: 637-52
5. Nakatsu MN and Hughes CCW 2008. An optimized three-dimensional in vitro model for the analysis of angiogenesis. Methods in Enzymology 443: 65-82
6. Hughes CCW 2008. Endothelial-stromal interactions in angiogenesis. Curr. Opinion Hematol. 15: 204-209.
Book Chapters:
1. Greenwood J, Luthert PJ, Pratt OE, Hazell AS, Hughes CCW. 1988. A supra-vital brain perfusion technique for the study of the blood-brain barrier, with special reference to leucine transport. In: Rakic L, Begley D, Davson H, Zlokovic B, eds. Peptide and Amino Acid Transport Mechanisms in the Central Nervous System. London:Macmillan, 317-331
2. Abbott NJ, Bundgaard M, Hughes CCW. 1989. Morphology of brain microvessels: a comparative approach. Bodensee Symposium: “Cerebral Microcirculation”. In: Hammersen F, Mensmer K. eds. Progress in Applied Microcirculation. Basel:Karger AG. 16:1-19
3. Pober JS, Hughes CCW, Savage COS, Schoen FJ, Libby P. 1991. Endothelial cell-lymphocyte interactions. In: Cellular and Cytokine Networks in Tissue Immunity. New York: Wiley-Liss, Inc, 183-188
4. Mestas J and Hughes CCW. 2006. The Mouse Trap: How Well do Mice Model Human Immunology? In: The Mouse in Biomedical Research. 2nd Ed. Elsevier, Inc..
5. Ghajar CM, Blevins KS, Hughes CCW, George SC, and Putnam AJ. 2010. Mesenchymal stem cells enhance angiogenesis in mechanically viable prevascularized tissues via early MMP upregulation. Ch. 23 In: Advances in Tissue Engineering, Vol. 1: Angiogenesis. New York: Mary Ann Liebert, Inc.
6. Chen X, Aledia AS, Ghajar CM, Griffith CK, Putnam AJ, Hughes CCW, and George SC. 2010 Prevascularization of a fibrin-based tissue construct accelerates the formation of functional anastomosis with host vasculature. Ch. 26 In: Advances in Tissue Engineering, Vol. 1: Angiogenesis. New York: Mary Ann Liebert, Inc.
Meeting Report:
1. Hughes CCW 1994. Inflammation in cardiovascular, lung, and blood diseases. “Frontiers in Basic Sciences” National Heart, Lung, and Blood Institute, NIH, November 29-30, 1993
Patents:
“Immunodeficient animal model for studying T cell-mediated immune responses” US5602305 Pober JS, Hughes CCW, Murray AG, Askenase PW, Petzelbauer P