Publications from the Hughes Lab

Cron RQ, Schubert LA, Lewis DB, and Hughes CCW. 1997. Protocols for consistent transient transfections of DNA into normal human and murine T lymphocytes. J. Immunol. Methods, 205: 145-150

Gonsky R, Deem RL, Hughes CCW, and Targan SR. 1998. Activation of the CD2 pathway in Lamina Propria T cells up-regulates functionally active AP-1 binding to the IL-2 promoter, resulting in mRNA transcription and IL-2 secretion. J. Immunol. 160: 4914-4922

Salazar Murphy LL, Mazanet MM, Taylor AC, Mestas J, and Hughes CCW. 1999 Single-cell analysis of costimulation by B cells, endothelial cells, and fibroblasts demonstrates heterogeneity in responses of CD4+ memory T cells. Cell. Immunol. 194: 150-161 (Pd f)

Taylor JA, Tewari K, Liao S-Y, Hughes CCW* and Villarreal LP. 1999 Immunohistochemical analysis, human papillomavirus DNA detection, hormonal manipulation, and exogenous gene expression of normal and dysplastic human cervical epithelium in severe combined immunodeficiency mice. J. Virol. 73: 5144-5148 * Joint senior author.

Mazanet MM, Neote K and Hughes CCW 2000. Expression of Interferon-inducible T cell alpha chemoattractant (I-TAC) by human endothelial cells is CsA-resistant and promotes T cell adhesion: Implications for CsA-resistant immune inflammation. J. Immunol. 164: 5383-88 (Pdf)

Tewari K, Taylor JA, Liao S-Y, DiSaia PJ, Burger RA, Monk BJ, Hughes CCW* and Villarreal LP 2000 Development and assessment of a general theory of cervical carcinogenesis utilizing a severe combined immunodeficiency murine-human xenograft model. Gynecol. Oncol. 77: 137-148 * Joint senior author.

Henderson AM, Wang S-J, Taylor AC, Aitkenhead M and Hughes CCW 2001 The bHLH transcription factor HESR1 regulates endothelial cell tube formation. J. Biol. Chem. 276: 6169-6176 (Pdf)

Mestas J and Hughes CCW. 2001. Endothelial cell costimulation of T cell activation through CD2-CD58 interactions involves lipid-raft aggregation J. Immunol. 167: 4378-4385 (Pdf)

Aitkenhead M, Wang S-J, Mestas J, Heard C and Hughes CCW. 2002. Identification of endothelial cell genes expressed in an in vitro model of angiogenesis: upregulation of ESM-1, big-h3 and NrCAM. Microvascular Research. 63: 159-171 (Pdf)

Mazanet MM and Hughes CCW. 2002. B7-H1 expression by activated endothelial cells mediates costimulation of T cell IL-10 production J. Immunol. 169: 3581-3588 (Pdf)

Salazar Murphy LL and Hughes CCW. 2002. Endothelial cells stimulate NFAT nuclear translocation in the presence of CsA: involvement of the wnt/GSK-3b pathway J. Immunol. 169: 3717-3725 (Pdf)

Taylor KT and Hughes CCW. 2002. Notch activation during endothelial cell tube formation in vitro targets the basic HLH transcription factor HESR1 and downregulates VEGFR-2/KDR expression. Microvascular Research. 64: 372-383 (Pdf)

Pedram A, Razandi M, Aitkenhead M, Hughes CCW and Levin ER. 2002 Integration of the non-genomic and genomic actions of estrogen: Membrane Initiated Signaling by Steroid (MISS) to transcription and cell biology. J. Biol. Chem. 277: 50768-50775 (Pdf)

Nakatsu MN, Sainson RCA, Aoto JN, Taylor KL, Aitkenhead M, Pérez del Pulgar S, Carpenter PM, and Hughes CCW. 2003 Angiogenic sprouting and capillary lumen formation modeled by human umbilical vein endothelial cells (HUVEC) in fibrin gels: the role of fibroblasts and Angiopoietin-1. Microvascular Research 66: 102-112 (Pdf)

Nakatsu MN, Sainson RCA, Pérez del Pulgar S, Aoto JN, Aitkenhead M, Taylor KL, Carpenter PM and Hughes CCW. 2003 VEGF121 and VEGF165 regulate blood vessel diameter through VEGFR-2 in an in vitro angiogenesis model. Laboratory Investigation 83: 1873-1885. (Pdf)

Mestas J, and Hughes CCW 2004. Of mice and not men: differences between mouse and human immunology. J. Immunol. 172: 2731-2738 (Pdf)

Griffith GK, Miller C, Sainson RCA, Calvert JW, Jeon NL, Hughes CCW, and George SC. 2005. Diffusion limits of an in vitro thick prevascularized tissue. Tissue Eng. 11: 257-266.

Sainson RCA, Aoto J, Nakatsu MN, Holderfield M, Conn E, Koller E, and Hughes CCW. 2005 Cell autonomous Notch signaling regulates endothelial cell branching and proliferation during vascular tubulogenesis. FASEB J 19: 1027-9

Mestas J, Crampton SC, Hori T and Hughes CCW. 2005 Endothelial cell costimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA. Int. Immunol. 17: 737-47

Payvandi F, Wu L, Naziruddin S, Haley M, Parton A, Schafer PH, Chen RS, Muller GW, Hughes CCW, and Stirling DI. 2005. Immunomodulatory drugs (IMiDs) increase the production of IL-2 from stimulated T cells by increasing PKC Activation And Enhancing the DNA binding activity of AP-1 but not NF-kB, OCT-1 or NF-AT. J. Interferon & Cytokine Res. 25: 604-16

Holderfield MT, Henderson Anderson AM, Kokubo H, Chin MT, Johnson RL and Hughes CCW. 2006 HESR1/CHF2 suppresses VEGFR2 transcription independent of binding to E-boxes. Biochem. Biophys. Res. Comm. 346: 637-648

Ghajar CM, Blevins KS, Hughes CCW, George SC, and Putnam AJ. 2006 Mesenchymal stem cells enhance angiogenesis in mechanically viable prevascularized tissues via early MMP upregulation. Tissue Engineering 10: 2875-2888.

Wu B, Crampton SP, and Hughes CCW. 2007. Wnt signaling induces MMP expression and regulates T cell transmigration. Immunity 26: 227-239.

Pedram A, Razandi M, Sainson RC, Kim JK, Hughes CCW, and Levin ER. 2007. A conserved mechanism for steroid receptor translocation to the plasma membrane. J. Biol. Chem. 282: 22278-88.

Nakatsu MN, Davis J, and Hughes CCW 2007 An optimized fibrin gel bead assay for the study of angiogenesis in vitro. J. Vis. Exp. (JoVE) 3: (Apr) doi:10.3791/186

Ghajar CM, Harris JW, Suresh V, Chen X, Jeon NL, Hughes CCW, Putnam AJ, and George SC. 2007. The Effect of Matrix Density on the Regulation of 3-D Capillary Morphogenesis. Biophys. J. 94: 1930-41

Sainson RCA, Johnston DA, Chu HC, Holderfield MT, Nakatsu MN, Crampton SP, Davis J, Conn E, and Hughes CCW. 2008 TNF Primes Endothelial Cells for Angiogenic Sprouting by Inducing a Tip Cell Phenotype. Blood 111: 4997-5007.

Lu L, Payvandi F, Wu L, Zhang L-H, Hariri RJ, Man H-W, Chen RS, Muller GW, Hughes CCW, Stirling DI, Schafer PH, and Bartlett JB. 2008 The anti cancer drug lenalidomide inhibits angiogenesis and metastasis via multiple inhibitory effects on endothelial cell function in normoxic and hypoxic conditions. Microvasc. Res. (in press)

Review Articles:

Mestas J, and Hughes CCW 2004. Of mice and not men: differences between mouse and human immunology. J. Immunol. 172: 2731-2738 (Pdf)

Holderfield MT and Hughes CCW. 2007 Crosstalk between VEGF, notch, and TGFb in vascular morphogenesis. Circ. Res 102: 637-52

Nakatsu MN and Hughes CCW 2008. An optimized three-dimensional in vitro model for the analysis of angiogenesis. Methods in Enzymology 443: 65-82

Hughes CCW 2008. Endothelial-stromal interactions in angiogenesis. Curr. Opinion Hematol. 15: 204-209.