DAVID M. GARDINER

Research Biologist
Department of Developmental and Cell Biology
and Developmental Biology Center
Room 4205 - BioSci II
University of California, Irvine
Irvine CA 92697-2275
Telephone/Fax: (949) 824-2792
Email: dmgardin@uci.edu
 

Education:

1980-1982: Post-doctoral training: University of California Davis
1976: Ph.D.: Scripps Institution of Oceanography, UCSD
1971: AB. Honors: Occidental College, Los Angeles
 

Research Programs and Expertise:

Molecular basis of pattern formation and growth control during limb development and regeneration.

Developmental and molecular mechanisms underlying amphibian malformations in North America.

Isolation and characterization of DNA and RNA, cDNA library construction and library screening, analysis of spatial and temporal patterns of gene expression, introduction and expression of transgenes in somatic cells, construction of retroviral expression vectors and retrovirus-mediated gene transfer, analysis of biological microstructure (histology, immunocytochemistry, and electron microscopy), cell culture, and microsurgery.

Positions Held:

1976-1978: Research Associate and Lecturer, Marine Biology, Department of Biology, Occidental College, Los Angeles, CA.
1978-1980: Visiting Assistant Professor of Biology/Associate Director of Marine Science, Occidental College, Los Angeles.
1980-1982; Visiting Assistant Professor of Zoology and Developmental Biology, UCD, Davis, CA
1982-1994: Assistant Research Biologist, Developmental Biology Center, UCI, Irvine, CA
1995-1996: Course Coordinator - Bio111L, Developmental and Cell Biology, UCI, Irvine, CA
1994-2000: Associate Research Biologist, Developmental Biology Center, UCI, Irvine, CA
1999-2000 Assistant Dean for Corporate Relations, School of Biological Sciences, UCI, Irvine, CA
2000 - present - Faculty Advisory Board, UCI-Santa Ana Teachers Institute
2000 - present - Orange County Science and Engineering Fair Board of Directors
2000 - present - Research Biologist, Department of Developmental and Cell Biology and Developmental Biology Center, UCI, Irvine, CA

Recent Publications:

Bridges, C., Little, E., Gardiner, D., Petty, J., and Huckins, J. (2001). Assessing the toxicity and teratogenicity of pond water in Minnesota to native amphibians (submitted).

Voss, S. R., Smith, J.J., Gardiner, D.M., and Parichy, D.M. (2001) Conserved Vertebrate Chromosome Segments in the Large Salamander Genome. Genetics. 158: 735-746.

Carlson, M.R.J., Komine, Y., Bryant, S.V., and Gardiner, D.M. (2001). Expression of Hoxb13 and Hoxc10 in developing and regenerating axolotl limbs and tails. Dev. Biol. 229, 396-406.

Roy, S., Gardiner, D.M. and Bryant, S.V. (2000). Vaccinia as a tool for functional analysis in regenerating limbs: ectopic expression of Shh. Dev. Biol. 218, 199-205.

Burkhart, J.G., Ankley, G., Bell, H., Carpenter, H., Fort, D., Gardiner, D., Gardner, H., Hale, R., Helgen, J.C., Jepson, P., Johnson, D., Lannoo, M., Lee, D., Lary, J., Levey, R., Magner, J., Meteyer, C., Shelby, M.D., and Lucier, G. (2000). Strategies for assessing the implications of malformed frogs for environmental health. Envir. Health Perspectives. 108, 83-90.

Yang, E.V., Nugas, C.A., Gardiner, D.M., Carlson, M. and Bryant, S.V. (1999). Expression of matrix metalloproteinase genes during axolotl limb regeneration. Dev Dynam. 216, 2-9.

Gardiner, D.M., Carlson, M.R.J.C. and Roy, S. (1999). Towards a functional analysis of limb regeneration. Sem. Cell & Dev. Biol. 10, 385-393.

Gardiner, D.M. and Hoppe, D.M. (1999). Environmentally-induced limb malformations in mink frogs (Rana septentrionalis). J. Exp. Zool. 284, 207-216.

Torok, M.A., Gardiner, D.M., Izpisua-Belmonte, J.C., and Bryant, S.V. (1999). Analysis of Sonic Hedgehog (shh) expression in developing and regenerating axolotl limbs. J. Exp. Zool. 284, 197-206.

Mills, A.A., Mills, M., Gardiner, D.M., Bryant, S.V., and Stanbridge, E.J. (1999). Expression of QM  during embryogenesis of the mouse. Differentiation 164, 161-171.

Carlson, M.R.J., Bryant, S.V. and Gardiner, D.M. (1998). Expression of Msx -2 during development, regeneration, and wound healing in axolotl limbs. J. Exp. Zool. 282, 715-723.

Gardiner, D.M. and Bryant, S.V. (1998). The Tetrapod Limb. In "Cellular and Molecular Basis of Regeneration: from invertebrates to humans", Ferretti, P. and Geraudie, J. (eds). John Wiley & Sons, Ltd. Chichester. pp. 187-205.

Gardiner, D.M., Torok, M.A., Mullen, L.M. and Bryant, S.V. (1998). Evolution of vertebrate limbs: Robust morphology and flexible development. Amer. Zool. 38, 659-671.

Torok, M.A., Gardiner, D.M., Shubin, N.H., and Bryant, S.V. (1998). Expression of HoxD genes in developing and regenerating axolotl limbs. Dev. Biol. 200, 225-233.

Metscher, B.D., Northcutt, R.G., Gardiner, D.M. and Bryant, S.V. (1997). Homeobox genes in axolotl lateral line: development and maintenance. Dev. Genes and Evol. 207, 287-295.

Ohsugi, K., Gardiner, D.M. and Bryant, S.V. (1997). Cell cycle length affects gene expression and pattern formation in limbs. Dev. Biol.,189:13-21.

Gardiner, D.M. and Bryant, S.V. (1996). Molecular mechanisms in the control of limb regeneration: the role of homeobox genes. Intl. J. Devel. Biol., 40: 797-805.

Mullen, L. M., Bryant, S.V., Torok, M.A., Blumberg, B. and Gardiner, D.M. (1996). Nerve Dependency of regeneration: The role of Distal-less and FGF signaling in amphibian limb regeneration. Development,122: 3487-3497.

Gardiner, D.M., Blumberg, B., Komine, Y. and Bryant, S.V. (1995). Regulation of HoxA expression in developing and regenerating axolotl limbs. Development, 121: 1731-1741.